The passive haemagglutination reaction with sonicated T. pallidum (Nichols strain) in the serodiagnosis of syphilis (TPHA)

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Authors

  • Voichitza LAZAR Institute of Medicine and Pharmacy Babes-Bolyai University, Romania
  • Florica KEVORKIAN Institute of Medicine and Pharmacy Babes-Bolyai University, Romania
  • Iuliu CĂPUŞAN Institute of Medicine and Pharmacy Babes-Bolyai University, Romania
  • Dezideriu AUSLANDER Institute of Medicine and Pharmacy Babes-Bolyai University, Romania
  • Eva VERESS Institute of Medicine and Pharmacy Babes-Bolyai University, Romania

Abstract

The preparation of the antigen for TPHA sonicated suspension of T. pallidum (Nichols strain) being so far a "secret" of the procedures, we have attempted to reproduce it by our own means of ultrasonic irradiation. The best results in agreement with the standard reactions for syphilis were obtained by sonicating T. pallidum suspensions for a duration of 20 min. by the following parameters of the ultrasonic field: frequency 1 MHz, intensity 0.9 W/cm^2. Using this method we obtained microfragments of 1-1.3 microns. The most reliable dilution for the antigen was 1/1000. The haernagglutination was performed in 30 patients with different forms of late syphilis after treatment. The results were in agreement with the clinical diagnosis, being more sensitive and consistent compared with the classical and standard reactions.

References

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